Through its own R&D activities, Microgen has developed an extensive range of diagnostic kits for the identification of bacteria isolated from a broad range of samples routinely submitted to clinical, food, environmental and veterinary laboratories. These products are based on two technology platforms, biochemical identification systems (Microgen TM ID) and latex agglutination (Microgen TM Latex).

MicrogenTM BIOCHEMICAL ID

The MicrogenTM range of biochemical identification systems currently includes systems for the following:

•Gram Negative Bacilli – Oxidase positive and negative. Download Product Information

•Listeria spp. – developed specially for the food industry and conforming to the requirements of all international food standards, this identification contains a unique micro-haemolysin test providing a fully self-contained identification system.

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•Bacillus spp. – developed primarily for the food and pharmaceutical industries for the identification of Bacillus spp. associated with food spoilage, environmental contamination and food poisoning.. This identification system comprises 24 biochemical tests specifically selected and formulated to provide the accurate identification of these organisms.

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•Staphylococcus spp. – intended for use in clinical, veterinary, food and environmental laboratories for the identification of all commonly encountered species of Staphylococci and some related species. This identification system employs 12 biochemical tests supplemented with 3 commonly employed screening tests to enable the accurate identification of these organisms within 24 hours of isolation on primary culture media.

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•Streptococcus spp. – intended for use in clinical, veterinary, food and environmental laboratories for the identification of all commonly encountered species of Streptococci and Enterococci. This identification system employs 13 biochemical tests supplemented with 2 commonly employed screening tests to enable the accurate identification of these organisms within 24 hours of isolation on primary culture media.

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The MicrogenTM BIOCHEMICAL ID systems are supported by the most comprehensive, powerful and easy-to-use, computerized identification system available. The combination of the appropriate MicrogenTM Biochemical ID System and the computerized identification system provides laboratories with a total package to meet their organism identification needs. MicrogenTM Biochemical ID System Software users are also provided with a FREE OFFER of database updates. All users of MicrogenTM Biochemical ID products and software should register as software users (see top of page) in order to be provided with their Software Update Password. Download product brochures (Requires Adobe Acrobat) Download technical data (Requires Adobe Acrobat) More Products Coming Soon

MICROGENTM LATEX AGGLUTINATION TESTS Employing highly specific antibodies developed by our own R&D Department, a range of rapid, quality latex agglutination kits are available for the confirmation of many of the key pathogens encountered in clinical laboratories, and food, environmental and water testing laboratories.

The benefits of these products include:

•Speed
•Simplicity
•Specificity
•Cost

Tests include:

•Salmonella spp. Download Product Information

•Campylobacter spp. Download Product Information

•C.difficile Download Product Information

•Staphylococcus aureus Download Product Information

•Streptococcal Grouping Download Product Information

•Legionella pneumophila Download Product Information

•E.coli O157 Download Product Information

•Listeria spp. Download Product Information

Consult VIROLOGY for Enteric Viral Antigen detection products in this range.

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PRE-PREPARED CHROMOGENIC MEDIA – AES LABORATOIRE, ONLY AVAILABLE IN THE UK

Microgen Bioproducts is the exclusive UK Distributor for the range of pre prepared agar plate media developed and manufactured by AES Laboratoire, France. The first of these products to be released into the UK is ALOA medium for the selective isolation of Listeria spp. and the presumptive identification of L. monocytogenes. The incorporation of the chromogenic substrate X-glucoside for the detection of beta-glucosidase demonstrates the presence of Listeria spp., whilst the detection of a specific phospholipase C enzyme produced by pathogenic Listeria spp. including L. monocytogenes is also achieved. Listeria spp. grow on this medium producing blue - green colonies, with the pathogenic species L. monocytogenes and L. ivanovii, producing similar coloured colonies surrounded by a characteristic opaque halo after 24 hours incubation at 37°C. Non Listeria spp. produce white colonies.

BENEFITS INCLUDE:

•Rapid - report negative results after 48 hours and positive results with confirmed indentifications after 72 hours.· AFNOR Approved (AES 10/3 09/00)
•Economical - save both material and labour costs
•Sensitive - low numbers of L.monocytogenes easily detected in mixed populations
•Specific - selective medium inhibits non Listeria organisms.

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ALSO AVAILABLE:

ASAP – Chromogenic pre preapred agar plate for the isolation of Salmonella spp.

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METISTAPH – Recommended for the isolation and presumptive identification of Methicillin Resistant Staphylococcus aureus from clincal samples.

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BLSE – Recommended for the isolation and presumptive identification of members of the family Enterobacteriaceae that produce Extended Spectrum Beta – Lactamase.

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INNOGENETICS®

INNO-LiPA® SYSTEMS

INNO-LiPA® Systems are a range of line probe assays for the detection and identification of Mycobacterial species

MYCOBACTERIA IDENTIFICATION The INNO-LiPA Mycobacteria v2 for the detection and identification of 16 Mycobacteria spp . from liquid or solid culture, is now available from Microgen Bioproducts Ltd. The assay is based on the reverse hybridisation principle where biotinylated DNA material is hybridised with specific oligonucleotide probes immobilised as parallel lines on membrane based strips. After hybridisation, streptavidin labelled with alkaline phosphatase is added and binds to any biotinylated hybrid present. Incubation with the chromogen BCIP/NBT results in a purple/brown precipitate. Amplification of the 16S-23S ribosomal RNA spacer region should be carried out using the primers provided. Amplified biotinylated product is subsequently hybridised using one typing strip onto which 22 DNA probe lines and 2 control lines are fixed. The INNO-LiPA assay can be automated using the AUTO-LiPA which runs the assay from sample incubation to completion. The Mycobacteria v2 line probe assay will identify the following species of Mycobacteria: M.kansasii (I-V probes), M.xenopi, M.gordonae, M.genavense, M.simiae, M.marinarum+ulcerans, M. celatum, MAIS complex, M.avium, M.intracellulare I&II probes, M.scrofulaceum, M.malmoense, M.haemophilum, M.chelonae (I-IV probes), M.fortuitum complex and M.smegmatis. NOW CE MARKED

INNO-LiPA Rif TB – line probe assay for the detection of Mycobacterium tuberculosis complex and its resistance to rifampicin. Rifampicin is one of the key drugs for treatment of tuberculosis and resistance to it may be considered as a marker for multi drug resistance. Rifampicin resistance is due to mutation in a short region of the ß-subunit of the RNA polymerase. The INNO-LiPA Rif. TB is a fast and convenient method of detecting mutations in the rpoB gene of Mycobacterium tuberculosis strains.

INNO-LIA Syphilis Score – line immunoassay for the confirmation of antibodies against Treponema pallidum in human serum and plasma. Three recombinant proteins (TpN47, TpN17 and TpN15) and one synthetic peptide (TmpA) are coated as discrete lines on a nylon strip with plastic backing.

NOT AVAILABLE FOR EXPORT

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See VIROLOGY and IMMUNOLOGY for additional products in this range.