Microgen Bioproducts are please to market the unique range of tissue culture products and virus identification products developed and manufcatured by Diagnostic Hybrids, USA.
CELL LINES AND DELIVERY SYSTEMS
DHI are the largest tissue culture cell manufacturer and distributor in the United States. What makes the products from DHI unique is both the nature of these products and the delivery systems they have made available which provide significant advantages in stock management.
Cell Line Delivery Systems
FreshCellsTM -
Diagnostic Hybrids, Inc. offers many conventional, single monolayer cell line products and Mixed cell lines for use in diagnosing and detecting viruses that cause human illness. FreshCells™ Singles are available in tube and shell vial format for conventional culture or flasks for re distribution to tubes or multi-well cluster plates. This format is delivered to the laboratory in a standard culture format which is ready to use. The disadvantage of this format is that like standard "in-house" manufactured cells, these cells will have a short shelf life (generally 1 - 2 weeks).
Frozen FreshCellsTM
Laboratories that are currently manufacturing their own cells but find it very costly and/ or time consuming will benefit from Frozen FreshCellsTM. In addition, laboratories who have considered producing their own cell lines and who have the neccessary basic facilities will also benefit from this cell delivery format.
Frozen FreshCellsTM are cell line concentrates provided in a frozen format. These are delivered with dry ice and transferred to -70oC where they can be stored for extended periods of time. Each aliquot of frozen cells cotains sufficient cells to produce 50 tubes or shell vials when reconstituted in Planting Medium.
Once reconstituted in Planting Medium and transferred into tubes or shell vials the cells are incubated and are ready for use within 2 - 3 days.
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ReadyCellsTM
FreshCellsTM provides the most innovative cell delivery system. Available as shell vials only, each vial contains a ready prepared cell monloayer in a frozen format. These vials are delivered to the laboratory in a frozen form and transferred into -70oC storage. As samples arrive in the laboratory the required number of vials are thawed, the transport medium removed and replaced with the ppropriate refeedmedium. The sample is then inoculated into the vial and processed.
FreshCellsTM are the most flexible delivery format for cell lines. No cell maintenance is required and no cell culture experience is necessary. This format is ideal for laboratories who wish to perform virus culture but who have no special cell culture facilities or limited expertise.
The advantages of Fozen FreshCellsTM and ReadyCellsTM include:
1. No cell line maintenance required.
2. Limited tissue culture experience necessary.
3. Cell culture tubes are always available.
4. Minimal or no wastage.
5. All refeeding media and accessory reagents are available.
Cell lines available
Apart from single cell lines available in tube or shell vial formats, DHI have developed a unique range of cell line format comprising specially developed mixed cell lines in the one container.
These innovative and patented products detect a larger number of viruses in a single container. Designed for broad virus detection and rapid turn-around time, these cultures make using shell vial technology more cost-effective and rapid than any other cell-based system can provide. Only available in a shell vial format.
Available Mixed Cell lines include:
R-MixTM
The R-Mix FreshCells™ product is a mixed monolayer of mink lung cells (strain Mv1Lu) and human Adenocarcinoma cells (strain A549). In combination, these cells support the detection of many viruses, in particular those of the respiratory group that includes Influenza A and B, RSV, Adenovirus, and Parainfluenza viruses 1,2, and 3.
R-Mix monolayers may be easily employed for detection of the complete range of respiratory viruses using a range of testing paradigms.
Example 1:
A single R-Mix vial is inoculated and cultured for 2 days. A suspension of the cell monolayer is produced, and a portion is used to make a smear for testing with a respiratory virus monoclonal antibody "cocktail". If positive by a respiratory virus screen, then multiple smears are prepared to differentiate which virus is present.
Example 2:
Three R-Mix vials are inoculated. One vial is blindly tested at day 1 post-inoculation using a respiratory virus screen; if Positive, a back-up vial is used to prepare a cell suspension for differentiating the specimen. If Negative at day 1, then the remaining cultures are incubated for 3 days and the process is repeated.
R-MixTM Too
Newly released - offers significant advantages in the culture of Influenza.
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Super E-MixTM
Super E-MixTM has combined two advanced technologies to create an advanced system for the detection and identification of Enteroviruses.
Super E-MixTM contains Super BGMK (Buffalo Green Monkey Kidney) cells that have been genetically transformed with the addition of Decay Acceleration Factor (DFA). Studies have validated that the virus infectivity is enhanced with these DFA transformed cells over non-transformed BGMK cells. Using DHI's proprietary Mixed Cell technology, A549 (Human Lung Carcinoma) cells have been added to the Super BGMK to provide the capability to detect all known culturable Enteroviruses in a single vial.
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Example:
Specimens are inoculated onto duplicate monolayers, centrifuged to enhance virus replication and incubated for 1 to 5 days. After incubation for 24 hours, one of the monolayers is fixed and stained using class-specific (Enterovirus) antibodies for the early detection of the virus by FIA (Fluorescence Immuno Assay). If the monolayer is negative for Enterovirusus, the remaining inoculated monolayer is cultured for 4 more days with examination each day for CPE (Cytopathic Effect). IF CPE is found on or before day 5 or if on day 5 no CPE is found, then the monolayer is fixed, stained using FIA and examined for fluorescence.
The advantages of Mixed Cell Technology:
1. Single tubes/ shell vials able to grow ALL of the targeted viruses.
2. Combination of 2 cell lines in mixed culture enhances culture sensitivity.
3. Combination of 2 cell lines in mixed culture decreases detection times.
DELIVERY SCHEDULES FOR FRESH CELLS AND FROZEN CELLS
Because of the relatively short shelf life of cell lines, Microgen Bioproducts have established a program by which customers can place orders knowing that the delivery will be made on a scheduled date.
For your convenience, the delivery schedules for Fresh Cells and Ready/Frozen Cells for 2007 and 2008 are provided below.
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| 13-Dec-06 |
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3-Jan-07 |
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| 17-Jan-07 |
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| 12-Dec-07 |
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| 16-Jan-08 |
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| Fresh Cells delivery Schedule 2007 |
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Fresh Cells delivery Schedule 2008 |
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VIRUS IDENTIFICATION
D3™ - DFA Respiratory Virus Screening & ID Kit
Direct Diagnostic detection , identification, and culture confirmation of Adenovirus, Influenza A and B viruses, Parainfluenza virus groups 1,2 and 3 and Respiratory Syncytial Virus
D3™ - DFA Respiratory Virus Screening & ID Kit identifiesthe seven major respiratory viruses (Adenovirus, Influenza A and B Parainfluenza 1, 2 and 3, and RSV) in 15 minutes from direct patient specimen materials or in incubated tissue cultures. Results of early detection (less than 48 hours from collection to result) are essential for specific patient therapy decisions.
The standard kit contains the screening reagent containing pooled monoclonal antibodies FITC Conjugated for the screening of direct patient specimens or cell culture preparations, PLUS specific monoclonal typing antibodies FITC Conjugated for the specific identification of preparations demonstrating positive fluorescence using the screening reagent.
Detectable
Infectious Agents
Adenovirus
Influenza A and B viruses
Parainfluenza virus groups 1,2 and 3
Respiratory Syncytial Virus
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DFA Monoclonal Antibody Reagents make staining a one-step process
15 Minute Incubation improves laboratory "time to result"
Bright Definition of Infected Cells using FITC labeling improves recognition of specific viral antigens
Liquid PBS/Detergent Concentrate eliminates time needed to dissolve crystalline materials
Evans Blue Counterstaining minimizes microscopist fatigue when dealing with negative samples
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Quick, Easy and Accurate
- For smears and scraped cells on microscope slides, add one to two drops. For shell vial monolayers, add 3-4 drops of DFA Screening Reagent
- Incubate at 37C for 15 minutes in a humidified chamber
- Wash stained cells with diluted PBS/detergent solution
- Add mounting fluid
- Examine microscopically at 200X or higher magnification using FITC filter system
Individual reagents are available separately.
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